Esterificação do ácido acético com álcool isoamílico na presença do catalisador enzimático

  • Nádia Ligianara Nyari Universidade Regional Integrada do Alto Uruguai e das Missões – URI – Erechim, RS, Brasil
  • Alessandro Rogerio Paulazzi Universidade Regional Integrada do Alto Uruguai e das Missões – URI - Erechim, RS, Brazil.
  • Raquel Vera Zamadei Universidade Regional Integrada do Alto Uruguai e das Missões – URI - Erechim, RS, Brazil
  • Jamile Zeni Universidade Regional Integrada do Alto Uruguai e das Missões – URI - Erechim, RS, Brazil
  • Rogério Marcos Dallago Universidade Regional Integrada do Alto Uruguai e das Missões – URI - Erechim, RS, Brazil
Palavras-chave: Candida antarctica Lipase B, Síntese de éster, Acetato de isoamila, Biocatálise

Resumo

A Lipase B da Candida antarctica imobilizada em esterificação catalisada por poliuretano de ácido acético com álcool isoamílico em sistema mecânico e ultrassônico em sistema isento de solventes foi estudada. A esterificação máxima do acetato de isoamila catalisador imobilizado utilizado foi a maior esterificação, 666,05 U/g com 19 ciclos de reuso após 360 minutos de tempo de reação no sistema mecânico e 1319,48 U/g foi observado, e 14 ciclos de reuso após 60 minutos de reação no sistema de ultrassom. Assim, o processo foi considerado eficiente com redução significativa do tempo de reação, baixos requisitos instrumentais e melhora no desempenho dos bioprocessos. Até o momento não havia estudos disponíveis na literatura aberta com relação à síntese de ésteres catalisada pela Lipase imobilizada em poliuretano como suporte no sistema de ultrassom. Além disso, considerada uma tecnologia ambientalmente correta e economicamente viável, pode ser usada em cosméticos, produtos farmacêuticos e indústria alimentícia.

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Referências

Welsh FW, Williams RE, Dawson KH. Lipase mediated synthesis of low molecular weight flavor esters. J Food Sci. 1990;55:1679.

Romero MD, Calvo L, Alba C, Daneshfar A, Ghaziaskar HS. Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in n-hexane. Enz Mic Technology. 2005;37:42.

World Flavors & Fragrances: Industry Study with Forecasts for 2016 & 2021. Available from: http:// www.freedoniagroup.com/DocumentDetails.aspx?Referrerid=FMBro&StudyID=2952. In: Food Flavors Market by Type (Chocolate, Vanilla, Fruits & Nuts, Others), Origin (Natural, Synthetic), Application (Beverages, Savory & Snacks, Bakery & Confectionery, Dairy & Frozen Products, Others), & by Region − Global Forecast to 2020. Available from: http://www.marketsandmarkets.com/Market-Reports/food- flavors-market-93115891.html

Dos Santos P, Meireles, MAA, Martínez J. Production of isoamyl acetate by enzymatic reactions in batch and packed bed reactors with supercritical CO2. J Supercrit Fluids. 2017;127:71.

Food Flavors Market By Type (Chocolate, Vanilla, Fruits & Nuts, Others), Origin (Natural, Synthetic), Application (Beverages, Savory & Snacks, Bakery & Confectionery, Dairy & Frozen Products, Others), & by Region – Global Forecast to 2020. Available from: http://www.marketsandmarkets.com/Market-Reports/food-flavors-market-93115891.html

Couto R, Vidinha P, Peres C, Ribeiro AS, Ferreira O, Oliveira MV, et al. Geranyl acetate synthesis in a packed-Bed reactor catalyzed by novozym in supercritical carbon dioxide and in supercritical ethane. Ind. Eng Chem Res. 2011;50:1938.

Dhake KP, Thakare DD, Bhanage BM. Lipase: a potential biocatalyst for the synthesis of valuable flavour and fragrance éster compounds. Flav Fragr J. 2013;28:71.

Tomin A, Hornyánszky G, Kupai K, Dorkó Z, Ürge L, Darvas F, et al. Lipase-catalyzed kinetic resolution of 2-methylene-substituted cycloalkanols in batch and continuous-flow modes. Process Bioch. 2010;45:859.

Hirata DB, Albuquerque TL, Rueda N, Virgen-Ortíz JJ, Tacias-Pascacio VG, Fernandez-Lafuente R. Evaluation of different immobilized lipases in transesterification reactions using tributyrin: Advantages of the heterofunctional octyl agarose beads. J Mol Catal B: Enz. 2016;133:117.

Ćorović M, Mihailović M, Banjanac K, Carević M, Milivojević A, Milosavić N, et al. Immobilization of Candida antarctica lipase B onto Purolite® MN102 and its application in solvent-free and organic media esterification. Bioprocess Biosystems Eng. 2017;40:23.

Garcia Galan C, Berenguer Murcia Á, Fernandez-Lafuente R, Rodrigues RC. Potential of different enzyme immobilization strategies to improve enzyme performance. Adv Synth Cat. 2011;353:2885.

Batistella L, Lerin L, Brugnerotto AJ, Danielli CM, Trentin A, Popiolski H, et al. Ultrasound-assisted lipase-catalyzed transesterification of soybean oil in organic solvent system. Ultras Sonoch. 2012;19:452.

Choudhury HA, Malani RS, Moholkar VS. Acid catalyzed biodiesel synthesis from Jatropha oil: mechanistic aspects of ultrasonic intensification. Chem Eng J. 2013;231:262.

Nyari NLD, Fernandes IA, Bustamante-Varga CE, Steffens CD, Zeni J, Dallago RM. In situ immobilization of Candida antarctica B lipase in polyurethane foam support. J Mol Catal B Enz. 2016;124:52.

Abou-Okeil A, El-Shafie A, El Zawahr MM. Ecofriendly laccase–hydrogen peroxide/ultrasound-assisted bleaching of linen fabrics and its influence on dyeing efficiency. Ultrason Sonoch. 2010;17:383.

Matte CR, Bordinhão C, Poppe JK, Rodrigues RC, Hertz PF, Ayub MA. Synthesis of butyl butyrate in batch and continuous enzymatic reactors using Thermomyces lanuginosus lipase immobilized in Immobead 150. J Mol Catal. B Enz. 2016;127:67.

Li L, Ji F, Wang J, Li Y, Bao Y. Esterification degree of fructose laurate exerted by Candida antarctica lipase B in organic solvents. Enzym Microbial Technol. 2015;69:46.

Azudin NY, Don MM, Shukor SRA. Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida antarctica Lipase B in a Solvent-Free System. J Food Quality. 2013;36:441.

Orellana-Coca C, Törnvall U, Adlercreutz D, Mattiasson B, Hatti-Kaul R. Chemo-enzymatic epoxidation of oleic acid and methyl oleate in solvent-free medium. Biocat. Biotransf. 2005;23:431.

Colombo TS, Mazutti MA, Di Luccio M, De Oliveira D, Olivera JV. Enzymatic synthesis of soybean biodiesel using supercritical carbon dioxide as solvent in a continuous expanded-bed reactor. J Superc Fluids. 2015;97:16.

Khan NR, Rathod VK. Enzyme catalyzed synthesis of cosmetic esters and its intensification: A review. Process Bioch. 2015;50:1793.

Liu YQ, Jin L, Shan Y, Liu W, Shen X. Wang, The effect of ultrasound on lipase-catalyzed hydrolysis of soy oil in solvent-free system. Ultrason. Sonoch. 2008;15:402.

Martins AB, Da Silva AM, Schein MF, Garcia-Galan C, Ayub MAZ, Fernandez-Lafuente R, et al. Comparison of the performance of commercial immobilized lipases in the synthesis of different flavor ésters. J Mol Cat B: Enz. 2014;105(1):18.

Carvalho AK, Faria EL, Rivaldi JD, Andrade GS, De Oliveira PC, De Castro HF. Performance of whole-cells lipase derived from Mucor circinelloides as a catalyst in the ethanolysis of non-edible vegetable oils under batch and continuous run conditions. Ind. Crops Prod. 2015;67:287.

Bansode SR, Rathod VK. Ultrasound assisted lipase catalyzed synthesis of isoamyl butyrate. Process Biochem. 2014;49:1297.

Waghmare GV, Vetal MD, Rathod VK. Ultrasound assisted enzyme catalyzed synthesis of glycerol carbonate from glycerol and dimethyl carbonate. Ultras Sonoch. 2015;22:311.

Poppe JK, Fernandez-Lafuente R, Rodrigues RC, Ayub MAZ. Enzymatic reactors for biodiesel synthesis: present status and future prospects. Biotechnol Adv. 2015;33:511.

Khan NR, Jadhav SV, Rathod VK. Lipase catalyzed synthesis of cetyl oleate using ultrasound: Optimization and kinetic studies. Ultras Sonoch. 2015;27:522.

Adewale P, Dumont MJ, Ngadi M. Enzyme-catalyzed synthesis and kinetics of ultrasonic assisted methanolysis of waste lard for biodiesel production. Chem Eng J. 2016;284:158164.

Michelin S, Penha FM, Sychoski MM, Scherer RP, Treichel H, Valério A, et al. Kinetics of ultrasound-assisted enzymatic biodiesel production from Macauba coconut oil. Renew Energ. 2015;76:388.

Publicado
17-12-2019
Como Citar
Nyari, N. L., Paulazzi, A. R., Zamadei, R. V., Zeni, J., & Dallago, R. M. (2019). Esterificação do ácido acético com álcool isoamílico na presença do catalisador enzimático. Evidência, 19(2), 225-242. https://doi.org/10.18593/eba.v19i2.20116