MOLECULAR ANALYSIS AND PROFILES OF ANTIBIOTICS RESISTANCE OF SALMONELLA ENTERITIDIS ISOLATES FROM POULTRY CHAIN PRODUCTION OF THE BRAZILIAN SOUTHERN REGION

  • Francielli Mello Ferri UNIVERSIDADE DO OESTE DE SANTA CATARINA-UNOESC
  • Jane Mary Lafayette Neves Gelinski UNOESC
  • César Milton Baratto UNOESC

Resumo

The agroindustrial sector of meats production and derivatives presents importance for the economic development in the Brazil's Southern States. Salmonella Enteritidis was isolated in all sectors of the productive chain of poultry and PCR-based techniques have demonstrated good applicability for identification and epidemiology studies of food pathogens. The aim of this work was to select molecular techniques with greatest potential to detect polymorphism of Salmonella Enteritidis strains, besides to determine the antibiotic resistance profile, and to find correlation between them. Sixty-eight Salmonella Enteritidis strains isolated from the poultry production chain in the southern States of Brazil were analyzed with the PAST program by UPGMA method and the similarity index Euclidean. The molecular technique RAPD, using the 23L primer, presented highest polymorphism, with 10 polymorphic groups and a Discriminatory Power of 0.96. For the resistance test, 75% of the isolates showed multiresistance, moreover the isolates were 100% resistant to erythromycin and 77% resistant to nalidixic acid. In conclusion, the RAPD molecular technique with the primer 23L have good applicability in epidemiological studies and the high incidence of mutiresistance isolates serves as an alert for the indiscriminate use of antibiotics in productions animals.

Biografia do Autor

Francielli Mello Ferri, UNIVERSIDADE DO OESTE DE SANTA CATARINA-UNOESC
Possui graduação em Ciências Biológicas pela Universidade do Contestado (2003). Especialista em Licenciamento, Auditoria e Perícia Ambiental. Mestranda em Ciência e Biotecnologia da UNOESC - Videira. Faz parte do grupo de pesquisa de Biotecnologia aplicada à agroindústria e saúde com ênfase em Bioprospecção, produção e processamento de matéria-prima e bioproduto. Atualmente desenvolve pesquisas no laboratório de Biologia Molecular da UNOESC, trabalha com técnicas moleculares como marcadores para polimorfismo em microrganismos.

Referências

BACK, A.; ISHIZUKA, M. M. Principais doenças de notificação obrigatória da Organização Mundial de Saúde Animal. São Paulo: Fundação Cargill, 239p, 2010.

TESSARI, E. N. C.; KANASHIRO, A. M. I.; STOPPA, G. F. Z.; LUCIANO, R. L.; DE CASTRO, A. G. M.; CARDOSO, A. L. S. P. Important aspects of Salmonella in the poultry industry and in public health. In: MAHMOUD, B. S. M. (Org.). Salmonella - A dangerous foodborne pathogen. 1. ed. Croatia: Intech, cap. 9, p. 181-199, 2012.

Publicado
20-12-2017
Como Citar
Ferri, F., Neves Gelinski, J., & Baratto, C. (2017). MOLECULAR ANALYSIS AND PROFILES OF ANTIBIOTICS RESISTANCE OF SALMONELLA ENTERITIDIS ISOLATES FROM POULTRY CHAIN PRODUCTION OF THE BRAZILIAN SOUTHERN REGION. Anais eletrônicos Do International Symposium on Science and Biotechnology. Recuperado de https://portalperiodicos.unoesc.edu.br/ISSB/article/view/16163