THE BEST TISSUE TO OBTAIN DNA FROM APPLE TREE USING CTAB EXTRACTION PROTOCOL

  • Luane Bosetto Universidade Estadual de Ponta Grossa
  • Thyana Lays Brancher Universidade Federal de Lavras
  • Cristiane Carlesso Universidade do Alto Vale do Rio do Peixe
  • Marcus Vinícius Kvitschal Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina
  • Maraisa Crestani Hawerroth Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina

Resumo

The DNA extraction is the key for all molecular biology procedures. In plants, this stage is absolutely affected by the tissue compounds, that are variable between the species. In apple tree, polyphenols are the main compounds that affects DNA quality and integrity. The aim of this work was to apply a protocol for DNA extraction using different apple tree tissues and to determine the best one to obtain high DNA quality. The protocol tested was an adaptation of CTAB, using leaf, bud, stem, apple skin and flesh. The experiment was carried out in a completely randomized design with five replicates, considering each tissue. To determinate the best quality of DNA was used de relation 260/280 nm and 260/230 nm. Quality parameters and DNA concentration were obtained with absorbance spectrophotometer. It was performed variance analysis and Tukey's test, with a significance level at 5 %. The DNA integrity was observed in agarose gel 0.8 %. The highest mean concentration were 374.53 ng/µL in stem and 395.58 ng/µL in bud tissue. The best quality (260/280 ≈ 2.0, 260/230 ≈ 1.8) was obtain with leaf, bud and stem, the same for DNA integrity. The best tissues to obtain DNA from apple tree with high concentration and quality were bud, stem and leaf, suggesting higher efficiency using this protocol.

Biografia do Autor

Thyana Lays Brancher, Universidade Federal de Lavras

Doutoranda em Biotecnologia Vegetal

MSc. Produção Vegetal

Biotecnologista Industrial

Publicado
20-12-2017
Como Citar
Bosetto, L., Brancher, T., Carlesso, C., Kvitschal, M., & Crestani Hawerroth, M. (2017). THE BEST TISSUE TO OBTAIN DNA FROM APPLE TREE USING CTAB EXTRACTION PROTOCOL. Anais eletrônicos Do International Symposium on Science and Biotechnology. Recuperado de https://portalperiodicos.unoesc.edu.br/ISSB/article/view/16158