PREVALENCE OF SALMONELLA ENTERICA HEIDELBERG IN THE PRODUCTIVE CHAIN OF BROILERS

  • Michael Scheffmacher UNOESC CURSO DE FARMÁCIA
  • Jane Lafayette Neves Gelinski Unoesc http://orcid.org/0000-0002-4378-5958
  • Fernanda Megiolaro UNOESC NÚCLEO BIOTECNOLÓGICO
  • Francielli De Mello Ferri UNOESC PROGRAMA DE MESTRADO EM CIÊNCIA E BIOTECNOLOGIA
  • Rafaela Ansiliero UNOESC CURSO DE BIOTECNOLOGIA INDUSTRIAL
  • César Milton Baratto UNOESC NÚCLEO BIOTECNOLÓGICO PROGRAMA DE MESTRADO EM CIENCIA E BIOTECNOLOGIA

Resumo

Salmonella enterica subsp. enterica Heidelberg is one of the most invasive serovar  and deserves attention due to its prevalence in the productive chain of borilers. This research analysed  the percentage of S. Heidelberg serotype samples in semi-solid medium (MSRV) and to evaluate the profile of antimicrobial susceptibility.  Isolation of samples from avian environment was performed from MSRV method  recommended by ISO 6579. Aliquots removed from the MSRV migration zone were inoculated in selective medium agar XLD and VB agar (DIFCO®) with incubation at 37ºC/24h. Were perfomed biochemical  and serological analysis of colonies as well molecular analysis based on PCR.  A total of 1506 samples were obtained, of which n=376 samples (24.96%) were positive for S. Heidelberg. From isolates, n=1130 did not migrate in MSRV (75.03%). However, n=62 (16.49%) confirmed as being S. Heidelberg. Isolates with migration in MSRV were  susceptible to all antimicrobials tested, whereas all at the group without migration (flagellar injury?) showed resistance to Ceftiofur and Neomycin.  It is early  to affirm the existence of a  relation  of using antimicrobials in PCB with the flagellar injury of the pathogen. However, monitoring should be performed, due we detect isolates resistant to at least two broad-spectrum antibiotics at the group of isolates with no ability to migrate in MSRV medium.

Keywords: Produtive chain. Salmonella. Antimicrobials. Flagella.

Biografia do Autor

Jane Lafayette Neves Gelinski, Unoesc

Professora

Mestre em Genética -UFRGS

Doutora em Ciência dos Alimentos-USP

Fernanda Megiolaro, UNOESC NÚCLEO BIOTECNOLÓGICO

Biotecnóloga

Mestre em Ciência e Biotecnologia

Francielli De Mello Ferri, UNOESC PROGRAMA DE MESTRADO EM CIÊNCIA E BIOTECNOLOGIA

BIÓLOGA

MESTRANDA EM CIENCIA E BIOTECNOLOGIA

Rafaela Ansiliero, UNOESC CURSO DE BIOTECNOLOGIA INDUSTRIAL

Aluna Curso de Biotecnologia Industrial

Bolsista de Iniciação Cientifica - CNPq

César Milton Baratto, UNOESC NÚCLEO BIOTECNOLÓGICO PROGRAMA DE MESTRADO EM CIENCIA E BIOTECNOLOGIA

BIÓLOGO

MESTRE E DOUTOR EM BIOLOGIA CELULAR E MOLECULAR

Publicado
20-12-2017
Como Citar
Scheffmacher, M., Gelinski, J. L. N., Megiolaro, F., Ferri, F. D. M., Ansiliero, R., & Baratto, C. M. (2017). PREVALENCE OF SALMONELLA ENTERICA HEIDELBERG IN THE PRODUCTIVE CHAIN OF BROILERS. Anais eletrônicos Do International Symposium on Science and Biotechnology. Recuperado de https://portalperiodicos.unoesc.edu.br/ISSB/article/view/16150