DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION OF KLEBSIELLA PNEUMONIAE KPC FRONT OF THE ESSENTIAL OIL OF MELALEUCA ALTERNIFOLIA

  • Alessandra Gioppo Universidade Alto Vale do Rio do Peixe- UNIARP.
  • Fernanda H. Lorenzon Pontifícia Universidade Católica do Paraná- PUC-PR
  • Emyr Hiago Bellaver Universidade Alto Vale do Rio do Peixe- UNIARP

Resumo

The essential oil of Melaleuca alternifolia (OTT) has antifungal, anti-inflammatory and antimicrobial effects reported in the literature that attributes this last to more than about 70% of terpenes present in its composition, and its mechanism of action consists in the breakdown of the membrane cytoplasmic activity leading to membrane protein damage, cytoplasmic coagulation, changes in electron fluxes and transport mechanisms and above all, the interruption of the proton motive force. In this century, the advent of multidrug-resistant microorganisms to available drugs has raised widespread concern, prompting industry and researchers to opt for alternative mechanisms in the treatment of some bacterial infections. The objective of the study was to evaluate the antibacterial properties of OTT against Klebsiella pneumoniae producing carbapenemase (KPC) and its standard strain K. pneumoniae ATCC 13883 by the Minimal Inhibitory Concentration (MIC) method by macrodilution and Spot-on-the-lawn. stipulated for KPC and K. pneumoniae ATCC to MIC in 0.25% OTT by the two methodologies, proving the antimicrobial activity of the oil and concluding that the mechanism of resistance to carbapenems does not influence the sensitivity to OTT.

Biografia do Autor

Alessandra Gioppo, Universidade Alto Vale do Rio do Peixe- UNIARP.
Naturóloga. Acadêmica do Curso de Farmácia da Universidade Alto Vale do Rio do Peixe- UNIARP.
Fernanda H. Lorenzon, Pontifícia Universidade Católica do Paraná- PUC-PR
Especialista em microbiologia. Pontifícia Universidade Católica do Paraná- PUC-PR
Emyr Hiago Bellaver, Universidade Alto Vale do Rio do Peixe- UNIARP
Biomédico patologista clínico e microbiologista. Mestre em Ciência e Biotecnologia. Professor do Núcleo de Ciências da Saúde da Universidade Alto Vale do Rio do Peixe- UNIARP.
Publicado
20-12-2017
Como Citar
Gioppo, A., Lorenzon, F., & Bellaver, E. (2017). DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION OF KLEBSIELLA PNEUMONIAE KPC FRONT OF THE ESSENTIAL OIL OF MELALEUCA ALTERNIFOLIA. Anais eletrônicos Do International Symposium on Science and Biotechnology. Recuperado de https://portalperiodicos.unoesc.edu.br/ISSB/article/view/16119